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Academic Press, New York. Naughton, G. , and Naughton, B. A. (1989). A physiological skin model for in vitro toxicity studies. In Progress in in Vitro Toxicology (A. M. ), Vol. 7, pp. 183-189. Liebert, New York. Nixon, G. , Tyson, C. , and Wertz, W. C. (1975). Interspecies comparisons of skin irritancy. Appl. Pharmacol. 31,481-590. Organization for Economic Cooperation and Development (OECD) (1992). OECD guidelines for testing of chemicals, Section 404, Acute Dermal Irritation/Corrosion, adopted July 17, 1992, Paris.

Is it appropriate? - - A r e we needlessly adding confounding variables (asking inadvertent or unwanted questions) ? w A r e there "lurking variables" present? There are undesired and not readily recognized differences which can affect results, such as different technicians observing different groups of animals. - - H o w large an effect will be considered biologically significant? This is a 2 Acute Toxicology Program: Study Design and Development 29 question which can only be resolved by reference to experience or historical control data.

Liebert, New York. , Akers, W. , McCreesh, A. , and Maibach, H. I. (1975).

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Acute toxicology

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